15, 2003 via Express Mail with the US post office, U.S. provisional patent application 60/463,668, Attorney Reference No. Preferably, the antibodies are specific to the spike (S) protein of the SARS virus.
The vector constructs were evaluated for expression 48 hr after transfection into 293 cells and COS7 cells. The antibodies are preferably monoclonal antibodies. Commonly used proteases include enterokinase, tobacco etch virus (TEV), thrombin, and factor X Accordingly, the invention further includes a SARS virus subunit vaccine comprising a fusion protein. The invention includes a polynucleotide sequence comprising one or more of the primer sequences identified in Table 32. In a further embodiment, the immunosuppressant compound is disclosed herein or set forth in Table 35. Numerous cloning vectors are known to those of skill in the art, and the selection of an appropriate cloning vector is a matter of choice.
The reaction was followed by TLC (CH The Pthalimido Pyrrole 4 (1 eq) was dissolved in ethanol and hydrazine (3 eq) at room temperature under nitrogen. Examples of useful promoters include those which are recognized by certain bacteriophage polymerases such as those from bacteriophage T3, T7 or SP6, or a promoter from Some of the reverse transcriptases suitable for use in the methods herein have an RNAse H activity, such as AMV reverse transcriptase. Bacterial host cells suitable for use in the invention include, for example, The SARS viral protein may be modified to facilitate bacterial recombinant expression. The method of claim 38, wherein the inactivation agent is β-propriolactone.40. These amidino and heterocycloamidino groups can be further substituted as will be apparent to those having skill in the organic and medicinal chemistry arts in conjunction with the disclosure herein. Culture conditions for the above cell types are well-described in a variety of publications, or alternatively culture medium, supplements, and conditions may be purchased commercially, such as for example, as described in the catalog and additional literature of Cambrex Bioproducts (East Rutherford, N.J.).
The proteins on the gel are stained or transferred to a membrane for Western blot analysis with sera from convalescent patients or alternatively murine or rabbit antisera generated against SARS virus. See., e.g., Edge, Once the coding sequences for the desired particle-forming polypeptides have been isolated or synthesized, they can be cloned into any suitable vector or replicon for expression. Suitable polymeric polyanionic stabilizers for use in the invention comprise either a single long chain or multiple cross linked chains; either type possessing multiple negative charges along the chains when in solution. For larger volumes, however, and for increased purity, ultrafiltration and sterile filtration steps will be used. An alignment of the polynucleotide sequence SEQ ID NO: 10084 to the encoded SEQ ID NO: 10149 is shown in The invention includes a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 10210, SEQ ID NO: 10211, SEQ ID NO: 10212, SEQ ID NO: 10213, SEQ ID NO: 10214 and SEQ ID NO: 10215. The invention further includes a polynucleotide encoding one or more of the fragments identified above. The HR1 and HR2 sequences of an MHV strain have been postulated to assemble into a thermostable, oligomeric, alphahelical rold-like complex, with the HR1 and HR2 helices oriented in an antiparallel manner. (1992) J.
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